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| Author(s): | Weigert, Martin Myers, Gene Kreysing, Moritz |
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| Abstract: | Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables to simulate operational light-sheet microscopes by combining data from 105–106 multiplexed and GPU-accelerated point-spread-function calculations. The wave-optical nature of these simulations leads to the faithful reproduction of spatially varying aberrations, diffraction artifacts, geometric image distortions, adaptive optics, and emergent wave optical phenom- ena, and renders image-formation in light-sheet microscopy computationally tractable. |
| Journal: | arXiv |
| Supplementary Data: |
lightsheet_sample_dn.tif (2.1 GB)lightsheet_sample_result.tif (401 MB)lightsheet_sample_signal.tif (1.1GB) |
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