| First Authors |
Jussi Helppi
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| Authors |
Jussi Helppi, Ronald Naumann, Marianne Asikainen, Jaakko Mononen, Oliver Zierau
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| Corresponding Authors |
Jussi Helppi
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| Last Authors |
Oliver Zierau
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| Journal Name |
Scandinavian Journal of Labooratory Animal Science (Scand J Lab Anim Sci) |
| Volume |
43 |
| Issue |
3 |
| Page Range |
1-5 |
| Open Access |
true |
| Print Publication Date |
2017-05-15 |
| Online Publication Date |
2017-05-15 |
| Abstract |
The impact of a novel bedding material (cotton cloth) on the reproductive performance (pregnancy rate and production of offspring) was studied in foster females used for producing transgenic mice. Embryos injected with DNA were transferred to pseudo-pregnant foster females housed under standard conditions (aspen bedding and nesting material). After embryo transfer, mice were divided between the experimental group (AGREBE cotton cloth) and control group (aspen bedding and nesting material). Pregnant mice were observed at day 15 after the transfer and the number of offspring was recorded on post-natal days 3 and 21. Altogether 116 foster mice were used as embryo recipients. Significantly more pregnancies were observed in the control group versus the experimental group: 43% and 19% of foster mice, respectively. Informal interviews with animal caretakers revealed a general dislike towards the cotton cloth (dirtier cages, mice often found on the plastic cage surface, difficult husbandry routines). The cotton cloth showed major signs of wear and tear after only a few weeks of usage. In conclusion, this study with female mice demonstrated that a cotton cloth cannot be recommended as a sole replacement for bedding and nesting material. |
| PDF |
Helppi_2017_6850.pdf
(592.2 kB)
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| Cover Image |
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| Affiliated With |
Biomedical Services, Transgenic Core Facility
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| Selected By |
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| Acknowledged Services |
Transgenic Core Facility, Biomedical Services
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| Publication Status |
Published |
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| Display Publisher Download Only |
false |
| Visible On MPI-CBG Website |
true |
| PDF Downloadable |
true |
| Created By |
thuem |
| Added Date |
2017-05-15 |
| Last Edited By |
thuem |
| Last Edited Date |
2017-06-06 16:22:40.275 |
| Library ID |
6850 |
| Document ID |
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| Entry Complete |
true |
| eDoc Compliant |
true |
| Include in Edoc Report |
true |
| In Pure |
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| Ready for eDoc Export |
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| Author Affiliations Complete |
false |
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