| First Authors | L Carine Stapel |
|---|---|
| Authors | L Carine Stapel, Coleman Broaddus, Nadine Vastenhouw |
| Corresponding Authors | |
| Last Authors | Nadine Vastenhouw |
| Journal Name | Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol) |
| Volume | 1649 |
| Issue | |
| Page Range | 143-162 |
| Open Access | false |
| Print Publication Date | 2018-01-01 |
| Online Publication Date | |
| Abstract | Single molecule fluorescence in situ hybridization (smFISH) is a method to visualize single mRNA molecules. When combined with cellular and nuclear segmentation, transcripts can be assigned to different cellular compartments resulting in quantitative information on transcript levels at subcellular resolution. The use of smFISH in zebrafish has been limited by the lack of protocols and an automated image analysis pipeline for samples of multicellular organisms. Here we present a protocol for smFISH on zebrafish cryosections. The protocol includes a method to obtain high-quality sections of zebrafish embryos, an smFISH protocol optimized for zebrafish cryosections, and a user-friendly, automated analysis pipeline for cell segmentation and transcript detection. The software is freely available and can be used to analyze sections of any multicellular organism. |
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| Affiliated With | Postdoc first author, Postdocs, Vastenhouw, Postdoc first female |
| Selected By | |
| Acknowledged Services | Media Technology and Outreach |
| Publication Status | Published |
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| DOI | 10.1007/978-1-4939-7213-5_9 |
| PubMed ID | 29130195 |
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| Display Publisher Download Only | false |
| Visible On MPI-CBG Website | true |
| PDF Downloadable | true |
| Created By | thuem |
| Added Date | 2017-11-30 |
| Last Edited By | herbst |
| Last Edited Date | 2021-05-27 17:51:01.753 |
| Library ID | 6984 |
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| Entry Complete | true |
| eDoc Compliant | true |
| Include in Edoc Report | true |
| In Pure | false |
| Ready for eDoc Export | false |
| Author Affiliations Complete | false |
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