| First Authors | Nereo Kalebic |
|---|---|
| Authors | Nereo Kalebic, Carlotta Gilardi, Barbara Stepien, Michaela Wilsch-Bräuninger, Katherine S. Long, Takashi Namba, Marta Florio, Barbara Langen, Benoit Lombardot, Anna Shevchenko, Manfred W Kilimann, Hiroshi Kawasaki, Pauline Wimberger, Wieland Huttner |
| Corresponding Authors | Wieland Huttner |
| Last Authors | Wieland Huttner |
| Journal Name | Cell stem cell (Cell Stem Cell) |
| Volume | 24 |
| Issue | 4 |
| Page Range | 535-550 |
| Open Access | false |
| Print Publication Date | 2019-04-04 |
| Online Publication Date | |
| Abstract | The evolutionary expansion of the mammalian neocortex (Ncx) is thought to be linked to increased proliferative capacity of basal progenitors (BPs) and their neurogenic capacity. Here, by quantifying BP morphology in the developing Ncx of mouse, ferret, and human, we show that increased BP proliferative capacity is linked to an increase in BP process number. We identify human membrane-bound PALMDELPHIN (PALMD-Caax) as an underlying factor, and we show that it drives BP process growth and proliferation when expressed in developing mouse and ferret Ncx. Conversely, CRISPR/Cas9-mediated disruption of PALMD or its binding partner ADDUCIN-γ in fetal human Ncx reduces BP process numbers and proliferation. We further show that PALMD-induced processes enable BPs to receive pro-proliferative integrin-dependent signals. These findings provide a link between BP morphology and proliferation, suggesting that changes in BP morphology may have contributed to the evolutionary expansion of the Ncx. |
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| Affiliated With | Biomedical Services, Huttner, Mass Spectrometry, Postdoc first author, Postdoc first male, Postdocs, Scientific Computing Facility, Shevchenko |
| Selected By | |
| Acknowledged Services | Genome Engineering Facility, Biomedical Services, Mass Spectrometry, Scientific Computing Facility, Light Microscopy Facility |
| Publication Status | Published |
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| DOI | 10.1016/j.stem.2019.02.017 |
| PubMed ID | 30905618 |
| WebOfScience Link | WOS:000463353000010 |
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| Display Publisher Download Only | false |
| Visible On MPI-CBG Website | true |
| PDF Downloadable | true |
| Created By | thuem |
| Added Date | 2019-04-03 |
| Last Edited By | herbst |
| Last Edited Date | 2021-05-03 17:31:46.681 |
| Library ID | 7370 |
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| Entry Complete | true |
| eDoc Compliant | true |
| Include in Edoc Report | true |
| In Pure | false |
| Ready for eDoc Export | false |
| Author Affiliations Complete | false |
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