First Authors | Iskra Yanakieva |
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Authors | Iskra Yanakieva, Anna Erzberger, Marija Matejčić, Carl D. Modes, Caren Norden |
Corresponding Authors | |
Last Authors | Caren Norden |
Journal Name | Journal of cell biology, The (J Cell Biol) |
Volume | 218 |
Issue | 10 |
Page Range | 3272-3289 |
Open Access | false |
Print Publication Date | 2019-10-07 |
Online Publication Date | |
Abstract | Correct nuclear position is crucial for cellular function and tissue development. Depending on cell context, however, the cytoskeletal elements responsible for nuclear positioning vary. While these cytoskeletal mechanisms have been intensely studied in single cells, how nuclear positioning is linked to tissue morphology is less clear. Here, we compare apical nuclear positioning in zebrafish neuroepithelia. We find that kinetics and actin-dependent mechanisms of nuclear positioning vary in tissues of different morphology. In straight neuroepithelia, nuclear positioning is controlled by Rho-ROCK-dependent myosin contractility. In contrast, in basally constricted neuroepithelia, a novel formin-dependent pushing mechanism is found for which we propose a proof-of-principle force generation theory. Overall, our data suggest that correct nuclear positioning is ensured by the adaptability of the cytoskeleton to cell and tissue shape. This in turn leads to robust epithelial maturation across geometries. The conclusion that different nuclear positioning mechanisms are favored in tissues of different morphology highlights the importance of developmental context for the execution of intracellular processes. |
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Affiliated With | CSBD, Modes, Norden, Predoc first author, Predoc first female |
Selected By | |
Acknowledged Services | Biomedical Services, Fish Facility, Scientific Computing Facility, Light Microscopy Facility |
Publication Status | Published |
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DOI | 10.1083/jcb.201901077 |
PubMed ID | 31420451 |
WebOfScience Link | WOS:000489119800012 |
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Display Publisher Download Only | false |
Visible On MPI-CBG Website | true |
PDF Downloadable | true |
Created By | thuem |
Added Date | 2019-08-30 |
Last Edited By | herbst |
Last Edited Date | 2022-06-15 13:55:36.627 |
Library ID | 7484 |
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Entry Complete | true |
eDoc Compliant | true |
Include in Edoc Report | true |
In Pure | false |
Ready for eDoc Export | false |
Author Affiliations Complete | false |
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