| First Authors | Antoine Graindorge |
|---|---|
| Authors | Antoine Graindorge, Inês Pinheiro, Anna Nawrocka, Allison C Mallory, Peter Tsvetkov, Noa Gil, Carlo Carolis, Frank Buchholz, Igor Ulitsky, Edith Heard, Mikko Taipale, Alena Shkumatava |
| Corresponding Authors | |
| Last Authors | Alena Shkumatava |
| Journal Name | Nature communications (Nat Commun) |
| Volume | 10 |
| Issue | 1 |
| Article Number | 5317 |
| Open Access | true |
| Print Publication Date | 2019-11-22 |
| Online Publication Date | |
| Abstract | Regulatory RNAs exert their cellular functions through RNA-binding proteins (RBPs). Identifying RNA-protein interactions is therefore key for a molecular understanding of regulatory RNAs. To date, RNA-bound proteins have been identified primarily through RNA purification followed by mass spectrometry. Here, we develop incPRINT (in cell protein-RNA interaction), a high-throughput method to identify in-cell RNA-protein interactions revealed by quantifiable luminescence. Applying incPRINT to long noncoding RNAs (lncRNAs), we identify RBPs specifically interacting with the lncRNA Firre and three functionally distinct regions of the lncRNA Xist. incPRINT confirms previously known lncRNA-protein interactions and identifies additional interactions that had evaded detection with other approaches. Importantly, the majority of the incPRINT-defined interactions are specific to individual functional regions of the large Xist transcript. Thus, we present an RNA-centric method that enables reliable identification of RNA-region-specific RBPs and is applicable to any RNA of interest. |
| Cover Image | |
| Affiliated With | Buchholz |
| Selected By | |
| Acknowledged Services | |
| Publication Status | Published |
| Edoc Link | |
| Sfx Link | |
| DOI | 10.1038/s41467-019-13235-w |
| PubMed ID | 31757954 |
| WebOfScience Link | WOS:000498186300001 |
| Alternative Full Text URL | |
| Display Publisher Download Only | false |
| Visible On MPI-CBG Website | true |
| PDF Downloadable | true |
| Created By | thuem |
| Added Date | 2019-12-02 |
| Last Edited By | thuem |
| Last Edited Date | 2019-12-04 10:24:13.512 |
| Library ID | 7563 |
| Document ID | |
| Entry Complete | true |
| eDoc Compliant | true |
| Include in Edoc Report | true |
| In Pure | |
| Ready for eDoc Export | |
| Author Affiliations Complete | false |
| Project Name | |
| Project URL | |
| Grant ID | |
| Funding Programme | |
| Funding Organisation |