First Authors | Matthäus Mittasch |
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Authors | Matthäus Mittasch, Vanna M. Tran, Manolo U. Rios, Anatol Fritsch, Stephen Enos, Beatriz Ferreira Gomes, Alec Bond, Moritz Kreysing, Jeffrey Woodruff |
Corresponding Authors | Moritz Kreysing, Jeffrey Woodruff |
Last Authors | Jeffrey Woodruff |
Journal Name | Journal of cell biology, The (J Cell Biol) |
Volume | 219 |
Issue | 4 |
Article Number | e201912036 |
Open Access | true |
Print Publication Date | 2020-04-06 |
Online Publication Date | |
Abstract | Centrosomes must resist microtubule-mediated forces for mitotic chromosome segregation. During mitotic exit, however, centrosomes are deformed and fractured by those same forces, which is a key step in centrosome disassembly. How the functional material properties of centrosomes change throughout the cell cycle, and how they are molecularly tuned, remain unknown. Here, we used optically induced flow perturbations to determine the molecular basis of centrosome strength and ductility in C. elegans embryos. We found that both properties declined sharply at anaphase onset, long before natural disassembly. This mechanical transition required PP2A phosphatase and correlated with inactivation of PLK-1 (Polo kinase) and SPD-2 (Cep192). In vitro, PLK-1 and SPD-2 directly protected centrosome scaffolds from force-induced disassembly. Our results suggest that, before anaphase, PLK-1 and SPD-2 respectively confer strength and ductility to the centrosome scaffold so that it can resist microtubule-pulling forces. In anaphase, centrosomes lose PLK-1 and SPD-2 and transition to a weak, brittle state that enables force-mediated centrosome disassembly. |
Mittasch_2020_7611.pdf (6.4 MB) | |
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Affiliated With | Kreysing, Predoc first author, Predoc first male |
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Acknowledged Services | Protein Expression, Light Microscopy Facility |
Publication Status | Published |
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DOI | 10.1083/jcb.201912036 |
PubMed ID | 32050025 |
WebOfScience Link | WOS:000525735400021 |
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Display Publisher Download Only | false |
Visible On MPI-CBG Website | true |
PDF Downloadable | true |
Created By | thuem |
Added Date | 2020-02-18 |
Last Edited By | herbst |
Last Edited Date | 2022-03-03 15:11:25.838 |
Library ID | 7611 |
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Entry Complete | true |
eDoc Compliant | true |
Include in Edoc Report | true |
In Pure | true |
Ready for eDoc Export | false |
Author Affiliations Complete | false |
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