| First Authors |
Christiane Iserman
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| Authors |
Christiane Iserman, Christine Desroches Altamirano, Ceciel Jegers, Ulrike Friedrich, Taraneh Zarin, Anatol Fritsch, Matthäus Mittasch, Antonio Domingues, Lena Hersemann, Marcus Jahnel, Doris Richter, Ulf-Peter Guenther, Matthias W. Hentze, Alan M Moses, Anthony Hyman, Günter Kramer, Moritz Kreysing, Titus Franzmann, Simon Alberti
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| Corresponding Authors |
Simon Alberti
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| Last Authors |
Simon Alberti
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| Journal Name |
Cell (Cell) |
| Volume |
181 |
| Issue |
4 |
| Page Range |
818-831 |
| Open Access |
true |
| Print Publication Date |
2020-05-14 |
| Online Publication Date |
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| Abstract |
Cells sense elevated temperatures and mount an adaptive heat shock response that involves changes in gene expression, but the underlying mechanisms, particularly on the level of translation, remain unknown. Here we report that, in budding yeast, the essential translation initiation factor Ded1p undergoes heat-induced phase separation into gel-like condensates. Using ribosome profiling and an in vitro translation assay, we reveal that condensate formation inactivates Ded1p and represses translation of housekeeping mRNAs while promoting translation of stress mRNAs. Testing a variant of Ded1p with altered phase behavior as well as Ded1p homologs from diverse species, we demonstrate that Ded1p condensation is adaptive and fine-tuned to the maximum growth temperature of the respective organism. We conclude that Ded1p condensation is an integral part of an extended heat shock response that selectively represses translation of housekeeping mRNAs to promote survival under conditions of severe heat stress. |
| PDF |
Iserman_2020_7664.pdf
(6 MB)
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| Cover Image |
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| Affiliated With |
Alberti, CSBD, Hyman, Kreysing, Postdocs, Predoc first author, Predoc first female, Scientific Computing Facility
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| Selected By |
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| Acknowledged Services |
Technology Development Studio TDS, Protein Expression, Scientific Computing Facility, Light Microscopy Facility, FACS Facility
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| Publication Status |
Published |
| Edoc Link |
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| Sfx Link |
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| DOI |
10.1016/j.cell.2020.04.009 |
| PubMed ID |
32359423 |
| WebOfScience Link |
WOS:000533623900009 |
| Alternative Full Text URL |
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| Display Publisher Download Only |
false |
| Visible On MPI-CBG Website |
true |
| PDF Downloadable |
true |
| Created By |
thuem |
| Added Date |
2020-05-05 |
| Last Edited By |
herbst |
| Last Edited Date |
2022-03-03 15:03:24.927 |
| Library ID |
7664 |
| Document ID |
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| Entry Complete |
true |
| eDoc Compliant |
true |
| Include in Edoc Report |
true |
| In Pure |
true |
| Ready for eDoc Export |
false |
| Author Affiliations Complete |
false |
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