First Authors | Tzer Han Tan |
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Authors | Tzer Han Tan, Aboutaleb Amiri, Irene Seijo-Barandiaran, Michael F Staddon, Anne Materne, Sandra Tomas, Charlie Duclut, Marko Popović, Anne Grapin-Botton, Frank Jülicher |
Corresponding Authors | Marko Popović, Anne Grapin-Botton, Frank Jülicher |
Last Authors | Frank Jülicher |
Journal Name | bioRxiv : the preprint server for biology (bioRxiv) |
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Article Number | doi: https://doi.org/10.1101/2022.09.29.510101 |
Open Access | true |
Print Publication Date | 2022-09-30 |
Online Publication Date | 2022-09-30 |
Abstract | Collective cell dynamics play a crucial role in many developmental and physiological contexts. While two-dimensional (2D) cell migration has been widely studied, how three-dimensional (3D) geometry and topology interplay with collective cell behavior to determine dynamics and functions remains an open question. In this work, we elucidate the biophysical mechanism underlying rotation in spherical tissues, a phenomenon widely reported both in vivo and in vitro. Using murine pancreas-derived organoids as a model system, we find that epithelial spheres exhibit persistent rotation, rotational axis drift and rotation arrest. Using a 3D vertex model, we demonstrate how the interplay between traction force and polarity alignment can account for these distinct rotational dynamics. Furthermore, our analysis shows that the spherical tissue rotates as an active solid and exhibits spontaneous chiral symmetry breaking. Using a continuum model, we demonstrate how the types and location of topological defects in the polarity field underlie this symmetry breaking process. Altogether, our work shows that tissue chirality can arise via topological defects in the pattern of cell traction forces, with potential implications for left-right symmetry breaking processes in morphogenetic events. |
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Affiliated With | CSBD, Grapin-Botton |
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Acknowledged Services | Biomedical Services, Light Microscopy Facility |
Publication Status | Published |
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DOI | 10.1101/2022.09.29.510101 |
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Display Publisher Download Only | false |
Visible On MPI-CBG Website | false |
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Created By | thuem |
Added Date | 2022-11-30 |
Last Edited By | thuem |
Last Edited Date | 2022-12-07 11:13:55.063 |
Library ID | 8481 |
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Entry Complete | true |
eDoc Compliant | true |
Include in Edoc Report | false |
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Ready for eDoc Export | false |
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