| First Authors |
Britta Weber
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| Authors |
Britta Weber, Garrett Greenan, Steffen Prohaska, Daniel Baum, Hans-Christian Hege, Thomas Müller-Reichert, Anthony A. Hyman, Jean-Marc Verbavatz
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| Corresponding Authors |
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| Last Authors |
Jean-Marc Verbavatz
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| Journal Name |
Journal of structural biology (J Struct Biol) |
| Volume |
178 |
| Issue |
2 SI |
| Page Range |
129-138 |
| Open Access |
false |
| Print Publication Date |
2012-05-01 |
| Online Publication Date |
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| Abstract |
The ability to rapidly assess microtubule number in 3D image stacks from electron tomograms is essential for collecting statistically meaningful data sets. Here we implement microtubule tracing using 3D template matching. We evaluate our results by comparing the automatically traced centerlines to manual tracings in a large number of electron tomograms of the centrosome of the early Caenorhabditis elegans embryo. Furthermore, we give a qualitative description of the tracing results for three other types of samples. For dual-axis tomograms, the automatic tracing yields 4% false negatives and 8% false positives on average. For single-axis tomograms, the accuracy of tracing is lower (16% false negatives and 14% false positives) due to the missing wedge in electron tomography. We also implemented an editor specifically designed for correcting the automatic tracing. Besides, this editor can be used for annotating microtubules. The automatic tracing together with a manual correction significantly reduces the amount of manual labor for tracing microtubule centerlines so that large-scale analysis of microtubule network properties becomes feasible. |
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| Supplementary Data |
Supplementary
Data
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| Affiliated With |
EM Facility, Hyman
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| Selected By |
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| Publication Status |
Published |
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| Sfx Link |
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| DOI |
10.1016/j.jsb.2011.12.004 |
| PubMed ID |
22182731 |
| WebOfScience Link |
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| Display Publisher Download Only |
false |
| Visible On MPI-CBG Website |
true |
| PDF Downloadable |
true |
| Created By |
thuem |
| Added Date |
2012-01-04 |
| Last Edited By |
smoha |
| Last Edited Date |
2015-05-07 19:09:25.627 |
| Library ID |
4629 |
| Document ID |
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| Entry Complete |
true |
| eDoc Compliant |
true |
| Include in Edoc Report |
true |
| In Pure |
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| Ready for eDoc Export |
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| Author Affiliations Complete |
false |
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