Combined RNAi and localization for functionally dissecting long non-coding RNAs
| First Authors | Debojyoti Chakraborty |
|---|---|
| Authors | Debojyoti Chakraborty, Dennis Kappei, Mirko Theis, Anja Nitzsche, Li Ding, Maciej Paszkowski-Rogacz, Vineeth Surendranath, Nicolas Berger, Herbert Schulz, Kathrin Saar, Norbert Hubner, Frank Buchholz |
| Corresponding Authors | Frank Buchholz |
| Last Authors | |
| Journal Name | Nature methods (Nat Methods) |
| Volume | 9 |
| Issue | 4 |
| Page Range | 360-362 |
| Open Access | false |
| Print Publication Date | 2012-02-12 |
| Online Publication Date | |
| Abstract | Whereas methods to comprehensively study cellular roles of protein-coding genes are available, techniques to systematically investigate long noncoding RNAs (lncRNAs), which have been implicated in diverse biological pathways, are limited. Here we report combined knockdown and localization analysis of noncoding RNAs (c-KLAN) that merges functional characterization and localization approaches to study lncRNAs. Using this technique we identified transcripts that regulate mouse embryonic stem cell identity. |
Chakraborty_2012_4649.pdf
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| Affiliated With | Buchholz, Postdocs |
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| Publication Status | Published |
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| DOI | 10.1038/nmeth.1894 |
| PubMed ID | 22327834 |
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| Created By | smoha |
| Added Date | 2012-01-20 |
| Last Edited By | smoha |
| Last Edited Date | 2013-03-12 12:42:44.578 |
| Library ID | 4649 |
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