Automated tracing of microtubules in electron tomograms of plastic embedded samples of Caenorhabditis elegans embryos.

First Authors Britta Weber
Authors Britta Weber, Garrett Greenan, Steffen Prohaska, Daniel Baum, Hans-Christian Hege, Thomas Müller-Reichert, Anthony A. Hyman, Jean-Marc Verbavatz
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Last Authors Jean-Marc Verbavatz
Journal Name Journal of structural biology (J Struct Biol)
Volume 178
Issue 2 SI
Page Range 129-138
Open Access false
Print Publication Date 2012-05-01
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Abstract The ability to rapidly assess microtubule number in 3D image stacks from electron tomograms is essential for collecting statistically meaningful data sets. Here we implement microtubule tracing using 3D template matching. We evaluate our results by comparing the automatically traced centerlines to manual tracings in a large number of electron tomograms of the centrosome of the early Caenorhabditis elegans embryo. Furthermore, we give a qualitative description of the tracing results for three other types of samples. For dual-axis tomograms, the automatic tracing yields 4% false negatives and 8% false positives on average. For single-axis tomograms, the accuracy of tracing is lower (16% false negatives and 14% false positives) due to the missing wedge in electron tomography. We also implemented an editor specifically designed for correcting the automatic tracing. Besides, this editor can be used for annotating microtubules. The automatic tracing together with a manual correction significantly reduces the amount of manual labor for tracing microtubule centerlines so that large-scale analysis of microtubule network properties becomes feasible.
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Affiliated With EM Facility, Hyman
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DOI 10.1016/j.jsb.2011.12.004
PubMed ID 22182731
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Created By thuem
Added Date 2012-01-04
Last Edited By smoha
Last Edited Date 2015-05-07 19:09:25.627
Library ID 4629
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