| First Authors | James Sáenz |
|---|---|
| Authors | James Sáenz, Daniel Grosser, Alexander S Bradley, Thibaut Lagny, Oksana Lavrynenko, Martyna Broda, Kai Simons |
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| Last Authors | Kai Simons |
| Journal Name | Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U.S.A.) |
| Volume | 112 |
| Issue | 38 |
| Page Range | 11971-11976 |
| Open Access | false |
| Print Publication Date | 2015-09-22 |
| Online Publication Date | |
| Abstract | The functionality of cellular membranes relies on the molecular order imparted by lipids. In eukaryotes, sterols such as cholesterol modulate membrane order, yet they are not typically found in prokaryotes. The structurally similar bacterial hopanoids exhibit similar ordering properties as sterols in vitro, but their exact physiological role in living bacteria is relatively uncharted. We present evidence that hopanoids interact with glycolipids in bacterial outer membranes to form a highly ordered bilayer in a manner analogous to the interaction of sterols with sphingolipids in eukaryotic plasma membranes. Furthermore, multidrug transport is impaired in a hopanoid-deficient mutant of the gram-negative Methylobacterium extorquens, which introduces a link between membrane order and an energy-dependent, membrane-associated function in prokaryotes. Thus, we reveal a convergence in the architecture of bacterial and eukaryotic membranes and implicate the biosynthetic pathways of hopanoids and other order-modulating lipids as potential targets to fight pathogenic multidrug resistance. |
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| Affiliated With | Postdocs, Simons, Shevchenko |
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| Publication Status | Published |
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| DOI | 10.1073/pnas.1515607112 |
| PubMed ID | 26351677 |
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| Display Publisher Download Only | false |
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| Created By | smoha |
| Added Date | 2015-09-10 |
| Last Edited By | smoha |
| Last Edited Date | 2015-10-01 16:27:33.567 |
| Library ID | 6288 |
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