| First Authors | Marco Y Hein |
|---|---|
| Authors | Marco Y Hein, Nina C Hubner, Ina Poser, Jürgen Cox, Nagarjuna Nagaraj, Yusuke Toyoda, Igor A Gak, Ina Weisswange, Jorg Mansfeld, Frank Buchholz, Anthony Hyman, Matthias Mann |
| Corresponding Authors | |
| Last Authors | Matthias Mann |
| Journal Name | Cell (Cell) |
| Volume | 163 |
| Issue | 3 |
| Page Range | 712-723 |
| Open Access | false |
| Print Publication Date | 2015-10-22 |
| Online Publication Date | |
| Abstract | The organization of a cell emerges from the interactions in protein networks. The interactome is critically dependent on the strengths of interactions and the cellular abundances of the connected proteins, both of which span orders of magnitude. However, these aspects have not yet been analyzed globally. Here, we have generated a library of HeLa cell lines expressing 1,125 GFP-tagged proteins under near-endogenous control, which we used as input for a next-generation interaction survey. Using quantitative proteomics, we detect specific interactions, estimate interaction stoichiometries, and measure cellular abundances of interacting proteins. These three quantitative dimensions reveal that the protein network is dominated by weak, substoichiometric interactions that play a pivotal role in defining network topology. The minority of stable complexes can be identified by their unique stoichiometry signature. This study provides a rich interaction dataset connecting thousands of proteins and introduces a framework for quantitative network analysis. |
| Cover Image | |
| Affiliated With | Buchholz, Hyman |
| Selected By | |
| Acknowledged Services | |
| Publication Status | Published |
| Edoc Link | |
| Sfx Link | |
| DOI | 10.1016/j.cell.2015.09.053 |
| PubMed ID | 26496610 |
| WebOfScience Link | |
| Alternative Full Text URL | |
| Display Publisher Download Only | false |
| Visible On MPI-CBG Website | true |
| PDF Downloadable | true |
| Created By | smoha |
| Added Date | 2015-11-16 |
| Last Edited By | smoha |
| Last Edited Date | 2016-02-29 14:32:41.72 |
| Library ID | 6374 |
| Document ID | |
| Entry Complete | true |
| eDoc Compliant | true |
| Include in Edoc Report | true |
| In Pure | |
| Ready for eDoc Export | |
| Author Affiliations Complete | false |
| Project Name | |
| Project URL | |
| Grant ID | |
| Funding Programme | |
| Funding Organisation |