| First Authors | Renata Zuber |
|---|---|
| Authors | Renata Zuber, Michaela Norum, Yiwen Wang, Kathrin Oehl, Nicole Gehring, Davide Accardi, Slawomir Bartozsewski, Jürgen Berger, Matthias Flötenmeyer, Bernard Moussian |
| Corresponding Authors | |
| Last Authors | Bernard Moussian |
| Journal Name | European journal of cell biology (Eur J Cell Biol) |
| Volume | 97 |
| Issue | 2 |
| Page Range | 90-101 |
| Open Access | false |
| Print Publication Date | 2018-03-01 |
| Online Publication Date | 2018-01-01 |
| Abstract | Lipids in extracellular matrices (ECM) contribute to barrier function and stability of epithelial tissues such as the pulmonary alveoli and the skin. In insects, skin waterproofness depends on the outermost layer of the extracellular cuticle termed envelope that contains cuticulin, an unidentified water-repellent complex molecule composed of proteins, lipids and catecholamines. Based on live-imaging analyses of fruit fly larvae, we find that initially envelope units are assembled within putative vesicles harbouring the ABC transporter Snu and the extracellular protein Snsl. In a second step, the content of these vesicles is distributed to cuticular lipid-transporting nanotubes named pore canals and to the cuticle surface in dependence of Snu function. Consistently, the surface of snu and snsl mutant larvae is depleted from lipids and cuticulin. By consequence, these animals suffer uncontrolled water loss and penetration of xenobiotics. Our data allude to a two-step model of envelope i.e. barrier formation. The proposed mechanism in principle parallels the events occurring during differentiation of the lipid-based ECM by keratinocytes in the vertebrate skin suggesting establishment of analogous mechanisms of skin barrier formation in vertebrates and invertebrates. |
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| Affiliated With | Light Microscopy Facility |
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| Publication Status | Published |
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| DOI | 10.1016/j.ejcb.2017.12.003 |
| PubMed ID | 29306642 |
| WebOfScience Link | WOS:000429397200002 |
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| Display Publisher Download Only | false |
| Visible On MPI-CBG Website | true |
| PDF Downloadable | true |
| Created By | thuem |
| Added Date | 2018-01-17 |
| Last Edited By | thuem |
| Last Edited Date | 2018-04-27 10:48:03.819 |
| Library ID | 7027 |
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| Entry Complete | true |
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| Include in Edoc Report | true |
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| Author Affiliations Complete | false |
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