| First Authors | Anna Bajur |
|---|---|
| Authors | Anna Bajur, K Venkatesan Iyer, Elisabeth Knust |
| Corresponding Authors | Elisabeth Knust |
| Last Authors | Elisabeth Knust |
| Journal Name | Journal of cell science (J Cell Sci) |
| Volume | 132 |
| Issue | 15 |
| Article Number | jcs228338 |
| Open Access | false |
| Print Publication Date | 2019-08-07 |
| Online Publication Date | |
| Abstract | During morphogenesis, epithelia undergo dynamic rearrangements, which requires continuous remodelling of junctions and cell shape, but at the same time mechanisms preserving cell polarity and tissue integrity. Apico-basal polarity is key for the localisation of the machinery that enables cell shape changes. The evolutionarily conserved Drosophila Crumbs protein is critical for maintaining apico-basal polarity and epithelial integrity. How Crumbs is maintained in a dynamically developing embryo remains largely unknown. Here, we applied quantitative fluorescence techniques to show that, during germ band retraction, Crumbs dynamics correlates with the morphogenetic activity of the epithelium. Genetic and pharmacological perturbations revealed that the mobile pool of Crumbs is fine-tuned by the actomyosin cortex in a stage-dependent manner. Stabilisation of Crumbs at the plasma membrane depends on a proper link to the actomyosin cortex via an intact FERM-domain-binding site in its intracellular domain, loss of which leads to increased junctional tension and higher DE-cadherin (also known as Shotgun) turnover, resulting in impaired junctional rearrangements. These data define Crumbs as a mediator between polarity and junctional regulation to orchestrate epithelial remodelling in response to changes in actomyosin activity.This article has an associated First Person interview with the first author of the paper. |
| Cover Image | |
| Affiliated With | Eaton, Knust, Postdoc first author, Postdocs, Postdoc first female |
| Selected By | |
| Acknowledged Services | Scientific Computing Facility, Light Microscopy Facility |
| Publication Status | Published |
| Edoc Link | |
| Sfx Link | |
| DOI | 10.1242/jcs.228338 |
| PubMed ID | 31300472 |
| WebOfScience Link | WOS:000481589200011 |
| Alternative Full Text URL | |
| Display Publisher Download Only | false |
| Visible On MPI-CBG Website | true |
| PDF Downloadable | true |
| Created By | thuem |
| Added Date | 2019-08-07 |
| Last Edited By | herbst |
| Last Edited Date | 2021-04-29 16:45:28.181 |
| Library ID | 7462 |
| Document ID | |
| Entry Complete | true |
| eDoc Compliant | true |
| Include in Edoc Report | true |
| In Pure | false |
| Ready for eDoc Export | false |
| Author Affiliations Complete | false |
| Project Name | |
| Project URL | |
| Grant ID | |
| Funding Programme | |
| Funding Organisation |