Defining the Identity and Dynamics of Adult Gastric Isthmus Stem Cells.

First Authors Seungmin Han
Authors Seungmin Han, Juergen Fink, David J. Jörg, Eunmin Lee, Min Kyu Yum, Lemonia Chatzeli, Sebastian R Merker, Manon Josserand, Teodora Trendafilova, Amanda Andersson-Rolf, Catherine Dabrowska, Hyunki Kim, Ronald Naumann, Ji-Hyun Lee, Nobuo Sasaki, Richard Lester Mort, Onur Basak, Hans Clevers, Daniel E Stange, Anna Philpott, Jong Kyoung Kim, Benjamin D Simons, Bon-Kyoung Koo
Corresponding Authors
Last Authors Bon-Kyoung Koo
Journal Name Cell stem cell (Cell Stem Cell)
Volume 25
Issue 3
Page Range 342-356
PubMed ID 31422913
WebOfScience Link
Open Access true
Print Publication Date 2019-09-05
Online Publication Date
Abstract The gastric corpus epithelium is the thickest part of the gastrointestinal tract and is rapidly turned over. Several markers have been proposed for gastric corpus stem cells in both isthmus and base regions. However, the identity of isthmus stem cells (IsthSCs) and the interaction between distinct stem cell populations is still under debate. Here, based on unbiased genetic labeling and biophysical modeling, we show that corpus glands are compartmentalized into two independent zones, with slow-cycling stem cells maintaining the base and actively cycling stem cells maintaining the pit-isthmus-neck region through a process of "punctuated" neutral drift dynamics. Independent lineage tracing based on Stmn1 and Ki67 expression confirmed that rapidly cycling IsthSCs maintain the pit-isthmus-neck region. Finally, single-cell RNA sequencing (RNA-seq) analysis is used to define the molecular identity and lineage relationship of a single, cycling, IsthSC population. These observations define the identity and functional behavior of IsthSCs.
Cover Image
Affiliated With Transgenic Core Facility
Selected By Transgenic Core Facility
Acknowledged Services
Publication Status Published
Edoc Link
Sfx Link
DOI 10.1016/j.stem.2019.07.008
Display Publisher Download Only false
Visible On MPI-CBG Website true
PDF Downloadable true
Created By naumann
Added Date 2019-08-22
Last Edited By thuem
Last Edited Date 2019-09-09 11:56:21.52
Library ID 7480
Document ID
Entry Complete true
eDoc Compliant true
Include in Edoc Report true
Author Affiliations Complete false