Active force generation shapes the metaphase spindle through a mechanical instability.

First Authors David Oriola
Authors David Oriola, Frank Jülicher, Jan Brugués
Corresponding Authors
Last Authors Jan Brugués
Journal Name bioRxiv (bioRxiv)
Volume
Issue
Article Number doi: 10.1101/2020.02.08.939868
PubMed ID
WebOfScience Link
Open Access true
Print Publication Date
Online Publication Date 2020-02-09
Abstract The metaphase spindle is a dynamic structure that segregates chromosomes during cell division. Recently, soft matter approaches have shown that the spindle behaves as an active liquid crystal. Still, it remains unclear how active force generation contributes to its characteristic spindle-like shape. Here, we combine theory and experiments to show that molecular motor driven forces shape the structure through a barreling-type instability. We test our physical model by titrating dynein activity in Xenopus egg extract spindles and quantifying the shape and microtubule orientation. We conclude that spindles are shaped by the interplay between surface tension, nematic elasticity and motor-driven active forces. Our study reveals how active force generation can mold liquid crystal droplets and it has implications on the morphology of non-membrane bound compartments demixed from the cytoplasm.
Cover Image
Affiliated With Brugues
Selected By
Acknowledged Services
Publication Status Published
Edoc Link
Sfx Link
DOI 10.1101/2020.02.08.939868
Display Publisher Download Only false
Visible On MPI-CBG Website true
PDF Downloadable true
Created By thuem
Added Date 2020-06-08
Last Edited By thuem
Last Edited Date 2020-06-08 10:08:46.334
Library ID 7691
Document ID
Entry Complete true
eDoc Compliant false
Include in Edoc Report false
Author Affiliations Complete false