| First Authors | Elisa Maria Rieckhoff |
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| Authors | Elisa Maria Rieckhoff, Frederic Berndt, Stefan Golfier, Franziska Decker, Maria Elsner, Keisuke Ishihara, Jan Brugués |
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| Last Authors | Jan Brugués |
| Journal Name | bioRxiv (bioRxiv) |
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| Article Number | https://doi.org/10.1101/2020.06.15.136937 |
| Open Access | true |
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| Online Publication Date | 2020-06-15 |
| Abstract | Cellular organellessuch as the mitotic spindleadjust their size to the dimensions of the cell. It is widely understood that spindle scaling is governed by regulation of microtubule polymerization.Here weusequantitative microscopyin living zebrafish embryos andXenopuseggextractsin combination with theoryto show that microtubule polymerization dynamics are insufficient to scale spindles and only contribute below a critical cell size. In contrast, microtubule nucleation governs spindle scaling for all cell sizes. We show that this hierarchical regulation arisesfrom the partitioning of a nucleation inhibitor to the cell membrane. Our results reveal that cells differentially regulatemicrotubule number and length using distinct geometric cues to maintain a functional spindle architecture over a large range of cell sizes. |
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| Affiliated With | Brugues, CSBD |
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| Publication Status | Published |
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| DOI | 10.1101/2020.06.15.136937 |
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| Display Publisher Download Only | false |
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| Created By | herbst |
| Added Date | 2020-10-28 |
| Last Edited By | herbst |
| Last Edited Date | 2022-02-18 15:30:42.544 |
| Library ID | 7833 |
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| Entry Complete | true |
| eDoc Compliant | false |
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