| First Authors | Elisabeth Kemter, Andreas Müller, Eckhard Wolf, Michele Solimena |
|---|---|
| Authors | Elisabeth Kemter, Andreas Müller, Martin Neukam, Anna Ivanova, Nikolai Klymiuk, Simone Renner, Kaiyuan Yang, Johannes Broichhagen, Mayuko Kurome, Valeri Zakhartchenko, Barbara Kessler, Klaus-Peter Knoch, Marc Bickle, Barbara Ludwig, Kai Johnsson, Heiko Lickert, Thomas Kurth, Eckhard Wolf, Michele Solimena |
| Corresponding Authors | Eckhard Wolf, Michele Solimena |
| Last Authors | Michele Solimena |
| Journal Name | Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U.S.A.) |
| Volume | 118 |
| Issue | 37 |
| Article Number | e2107665118 |
| Open Access | true |
| Print Publication Date | 2021-09-14 |
| Online Publication Date | 2021-09-14 |
| Abstract | β cells produce, store, and secrete insulin upon elevated blood glucose levels. Insulin secretion is a highly regulated process. The probability for insulin secretory granules to undergo fusion with the plasma membrane or being degraded is correlated with their age. However, the molecular features and stimuli connected to this behavior have not yet been fully understood. Furthermore, our understanding of β cell function is mostly derived from studies of ex vivo isolated islets in rodent models. To overcome this translational gap and study insulin secretory granule turnover in vivo, we have generated a transgenic pig model with the SNAP-tag fused to insulin. We demonstrate the correct targeting and processing of the tagged insulin and normal glycemic control of the pig model. Furthermore, we show specific single- and dual-color granular labeling of in vivo-labeled pig pancreas. This model may provide unprecedented insights into the in vivo insulin secretory granule behavior in an animal close to humans. |
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| Affiliated With | Technology Development Studio TDS |
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| Acknowledged Services | Light Microscopy Facility, Antibody Facility |
| Publication Status | Published |
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| DOI | 10.1073/pnas.2107665118 |
| PubMed ID | 34508004 |
| WebOfScience Link | WOS:000705153400007 |
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| Visible On MPI-CBG Website | true |
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| Created By | herbst |
| Added Date | 2021-09-13 |
| Last Edited By | herbst |
| Last Edited Date | 2022-02-28 13:28:18.469 |
| Library ID | 8180 |
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| Entry Complete | true |
| eDoc Compliant | true |
| Include in Edoc Report | true |
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| Author Affiliations Complete | false |
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