| First Authors | Mark Skamrahl |
|---|---|
| Authors | Mark Skamrahl, Justus Schünemann, Markus Mukenhirn, Hongtao Pang, Jannis Gottwald, Marcel Jipp, Maximilian Ferle, Angela Rübeling, Tabea A Oswald, Alf Honigmann, Andreas Janshoff |
| Corresponding Authors | Andreas Janshoff |
| Last Authors | Andreas Janshoff |
| Journal Name | Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U.S.A.) |
| Volume | 120 |
| Issue | 15 |
| Article Number | e2213186120 |
| Open Access | true |
| Print Publication Date | 2023-04-11 |
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| Abstract | Cellular sorting and pattern formation are crucial for many biological processes such as development, tissue regeneration, and cancer progression. Prominent physical driving forces for cellular sorting are differential adhesion and contractility. Here, we studied the segregation of epithelial cocultures containing highly contractile, ZO1/2-depleted MDCKII cells (dKD) and their wild-type (WT) counterparts using multiple quantitative, high-throughput methods to monitor their dynamical and mechanical properties. We observe a time-dependent segregation process governed mainly by differential contractility on short (<5 h) and differential adhesion on long (>5 h) timescales. The overly contractile dKD cells exert strong lateral forces on their WT neighbors, thereby apically depleting their surface area. Concomitantly, the tight junction-depleted, contractile cells exhibit weaker cell-cell adhesion and lower traction force. Drug-induced contractility reduction and partial calcium depletion delay the initial segregation but cease to change the final demixed state, rendering differential adhesion the dominant segregation force at longer timescales. This well-controlled model system shows how cell sorting is accomplished through a complex interplay between differential adhesion and contractility and can be explained largely by generic physical driving forces. |
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| Affiliated With | Honigmann |
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| Publication Status | Published |
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| DOI | 10.1073/pnas.2213186120 |
| PubMed ID | 37011207 |
| WebOfScience Link | WOS:001025193500003 |
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| Created By | thuem |
| Added Date | 2023-04-11 |
| Last Edited By | thuem |
| Last Edited Date | 2023-08-10 14:50:58.996 |
| Library ID | 8521 |
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