| First Authors | Natalia P Smirnova |
|---|---|
| Authors | Natalia P Smirnova, Sergey V. Ponomartsev, Tharvesh M. Liyakat Ali, Max Lycke, Brian K. Chung, Jonas Ogaard, Espen Melum, Jesse V Veenvliet, Stefan Krauss |
| Corresponding Authors | |
| Last Authors | Stefan Krauss |
| Journal Name | bioRxiv : the preprint server for biology (bioRxiv) |
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| Article Number | doi: https://doi.org/10.1101/2025.11.09.687163 |
| Open Access | true |
| Print Publication Date | |
| Online Publication Date | 2025-11-12 |
| Abstract | During mouse embryogenesis, the interactions between the epiblast and extraembryonic endoderm are critical for germ layer specification and body plan development. Gastruloids recapitulate aspects of gastrulation in the absence of morphogenic signals from the extraembryonic environment. This favors a predominantly posteriorized and dorsalized phenotype with a limited representation of embryonic lineages. Here, we develop and employ a co-aggregation (aggregoid) approach combining embryonic and extraembryonic endoderm-like cells to mimic spatial interactions in developing mouse embryos. The obtained embryo models show the appearance of node and notochord, enriched endoderm populations, increased mesoderm diversity including cardiopharyngeal lineages and vascular endothelium in an overall anteriorized and ventralized phenotype. The work delineates a versatile strategy for refining stem cell-based embryo models to achieve specific morphotypes |
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| Affiliated With | Veenvliet |
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| Publication Status | Published |
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| DOI | 10.1101/2025.11.09.687163 |
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| Alternative Full Text URL | https://doi.org/10.1101/2025.11.09.687163 |
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| Created By | thuem |
| Added Date | 2026-01-09 |
| Last Edited By | thuem |
| Last Edited Date | 2026-01-09 11:25:19.256 |
| Library ID | 9119 |
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