Tracking single particles and elongated filaments with nanometer precision.
| First Authors | Felix Ruhnow |
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| Authors | Felix Ruhnow, David Zwicker, Stefan Diez |
| Corresponding Authors | Stefan Diez |
| Last Authors | Stefan Diez |
| Journal Name | Biophysical journal (Biophys J) |
| Volume | 100 |
| Issue | 11 |
| Page Range | 2820-2828 |
| Open Access | false |
| Print Publication Date | 2011-06-01 |
| Online Publication Date | |
| Abstract | Recent developments in image processing have greatly advanced our understanding of biomolecular processes in vitro and in vivo. In particular, using Gaussian models to fit the intensity profiles of nanometer-sized objects have enabled their two-dimensional localization with a precision in the one-nanometer range. Here, we present an algorithm to precisely localize curved filaments whose structures are characterized by subresolution diameters and micrometer lengths. Using surface-immobilized microtubules, fluorescently labeled with rhodamine, we demonstrate positional precisions of ∼2 nm when determining the filament centerline and ∼9 nm when localizing the filament tips. Combined with state-of-the-art single particle tracking we apply the algorithm 1), to motor-proteins stepping on immobilized microtubules, 2), to depolymerizing microtubules, and 3), to microtubules gliding over motor-coated surfaces. |
Ruhnow_2011_4494.pdf
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| Affiliated With | Diez |
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| Publication Status | Published |
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| DOI | 10.1016/j.bpj.2011.04.023 |
| PubMed ID | 21641328 |
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| Added Date | 2011-06-27 |
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| Last Edited Date | 2011-07-09 03:05:25.008 |
| Library ID | 4494 |
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