| First Authors |
Anastasia Solomatina
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| Authors |
Anastasia Solomatina, Yannis Kalaidzidis, Alice Cezanne, Karen Soans, Caren Norden, Marino Zerial, Ivo F. Sbalzarini
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| Corresponding Authors |
Ivo F. Sbalzarini
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| Last Authors |
Ivo F. Sbalzarini
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| Conference Proceedings Volume Title |
ISBI 2021, IEEE International Symposium on Biomedical Imaging (ISBI) |
| Series Title |
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| Conference Name |
2021 IEEE 18th International Symposium on Biomedical Imaging (ISBI) |
| Conference Location |
Nice, France |
| Conference Start Date |
2021-04-13 |
| Conference End Date |
2021-04-16 |
| Chapter Number |
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| Publisher |
IEEE |
| Conference Proceedings Editors |
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| ISBN |
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| First Page |
1150 |
| Last Page |
1154 |
| Print Publication Date |
2021-05-25 |
| Online Publication Date |
2021-05-25 |
| Abstract |
We present a novel method for model-based segmentation of extended, blob-like objects on curved surfaces. Our method addresses several challenges arising when imaging curved biological membrane, such as out-of-membrane signal and geometry-induced background variations. We use a particle-based reconstruction of the membrane geometry, moment-conserving intensity interpolation from pixels to surface particles, and model-based in-surface segmentation. Our method denoises and deconvolves images, corrects for background variations, and quantifies the number, size, and intensity of segmented objects. We benchmark the accuracy of the method and present two applications to (1) neuroepithelial focal adhesion sites during optic cup morphogenesis in zebrafish and (2) reconstituted membrane domains bearing the small GTPase Rab5 on spherical beads. |
| PDF |
Solomatina_2021_8040.pdf
(3.4 MB)
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| Cover Image |
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| Affiliated With |
CSBD, Sbalzarini, Zerial, Norden
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| Selected By |
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| Publication Status |
Published |
| Edoc Link |
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| Sfx Link |
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| DOI |
10.1109/ISBI48211.2021.9434038 |
| PubMed ID |
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| WebOfScience Link |
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| Display Publisher Download Only |
true |
| Visible On MPI-CBG Website |
true |
| PDF Downloadable |
false |
| Created By |
sbalzari |
| Added Date |
2021-05-06 |
| Last Edited By |
thuem |
| Last Edited Date |
2022-05-24 11:35:29.748 |
| Library ID |
8040 |
| Document ID |
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| Entry Complete |
true |
| eDoc Compliant |
true |
| Include in Edoc Report |
true |
| In Pure |
true |
| Ready for eDoc Export |
false |
| Author Affiliations Complete |
false |
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